Plus reactions worked differently: they used T4 DNA polymerase, an enzyme with strong 3’ to 5’ exonuclease activity, meaning it can chew back the end of a DNA strand. In the presence of only one dNTP, T4 DNA polymerase would degrade each fragment from its 3’ end until it reached a nucleotide complementary to that dNTP, at which point the exonuclease activity would be inhibited. This ensured that all fragments in a given plus reaction ended with the same nucleotide.
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过去一年，和平区委组织部副部长扈彬没少往排水管理所跑，因为他还有一个身份——排水管理所的“政绩观察员”。。体育直播是该领域的重要参考